Iimveliso
Umkhuhlane A Antibody ELISA Kit
Umkhuhlane A Antibody ELISA Kit
| Isishwankathelo | Ukufunyanwa kwe-Antibody ethile yoMkhuhlane Ukuxilongwa kwe-immune kunye ne-serological yosulelo kwi-Avian, iihagu kunye ne-Equus. |
| Umgaqo | Umkhuhlane Ikhithi ye-antibody Elisa isetyenziselwa ukufumanisa i-antibody ethile ngokuchasene ne-Iumkhuhlane iNtsholongwane (Flu A) kwiserum, yokuhlola i-antibody emva komkhuhlane Amajoni omzimbakunye nokuxilongwa kwe-serological yosulelo kwi-Avian, iihagu kunye Equus.
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| Iinjongo zokuFumana | Umkhuhlane I-antibody |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
Intsholongwane ye-Influenza A yintsholongwane ebangela umkhuhlane kwiintaka kunye nezinye izilwanyana ezincancisayo.Yintsholongwane ye-RNA, ii-subtypes eziye zahlukaniswa kwiintaka zasendle.Ngamanye amaxesha, isasazeka kwiintaka zasendle ukuya kwinkukhu, nto leyo inokukhokelela kwizifo ezinzima, ukuqhambuka, okanye umkhuhlane wabantu.
Umgaqo woVavanyo
Le kit isebenzisa ibhloko indlela ye-ELISA, i-antigen ye-FluA ifakwe ngaphambili kwi-microplate.Xa uvavanya, yongeza isampulu yeserum ehlanjululweyo, emva kokufukanyelwa, ukuba kukho Umkhuhlane I-antibody ethile, iya kudibanisa kunye ne-antigen efakwe ngaphambili, ilahle i-antibody engadibaniyo kunye namanye amacandelo ngokuhlamba;Emva koko yongeza i-enzyme ebhalwe ukuba yi-anti-Flu I-antibody ye-monoclonal, i-antibody kwibloko yesampulu indibaniselwano ye-monoclonal antibody kunye ne-antigen eqatywe ngaphambili;lahla i-enzyme engadityaniswanga idibanisa kunye nokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
