Iimveliso
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
| Isishwankathelo | USe ukufumanisa i-antibody ethile ngokuchasene ne-EDS76 kwiserum ngokomgangatho. |
| Umgaqo | I-Eggs Drop Syndrome 1976 virus(EDS76) ikiti ye-Ab Elisa isetyenziselwa ukufumanisa i-antibody ethile echasene ne-EDS76 kwiserum ngokomgangatho.Ukubeka iliso kwi-antibody emva kwe-EDS76 yokuxilonga i-immune kunye ne-serological yosulelo kwi-Avian. |
| Iinjongo zokuFumana | Ai-antibody ngokuchasene ne-EDS76 kwi-serum |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
I-Egg Drop Syndrome (EDS-76) sisifo esosulelayo esinamandla esibangelwa liqela le-adenovirus III ye-Adenoviridae Avian Virus genus ene-hemagglutination.Kwezinye iifama zenkukhu, ukuveliswa kwamaqanda amaninzi eenkuku ngesiquphe kwancipha, kwaza kwaveliswa amaqanda akhubazekileyo anjengamaqanda amaqokobhe athambileyo, amaqanda angenaqokobhe, namaqanda abhityileyo.Ikhosi yonke yesifo ithatha iiveki ezi-5-6, emva koko izinga lemveliso yeqanda liyenyuka ngokuthe ngcembe, kodwa kunzima ukufikelela kwinqanaba ngaphambi kokuhla.
Umgaqo woVavanyo
Le khithi isebenzisa indlela ye-ELISA engathanga ngqo, i-antigen ye-EDS76 ecocekileyo iqatywe ngaphambili kwi-enzyme micro-well strips.,ukuba kukho i-EDS76 intsholongwane engqamene nomntu othile, iya kudityaniswa ne-antigen yangaphambili, ilahle i-antibody engadityaniswanga kunye nezinye izinto ngokuhlamba;emva koko wongeze i-enzyme ebhalwe i-anti-EDS76 virus monoclonal antibody, emva koko indibaniselwano ye-monoclonal antibody kunye ne-antigen efakwe ngaphambili;lahla i-enzyme edibeneyo edibeneyo kunye nokuhlamba;Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ohlaza okwesibhakabhaka nge-Enzyme catalysis ikwinqanaba eliguquguqukayo lomxholo we-antibody kwisampulu, sebenzisa umfundi we-ELISA kwi-450nm wavelenth ukulinganisa ukufunxa Ixabiso kwimithombo yokusabela emva kokongeza isisombululo sokumisa.
ukumisa ukusabela.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
