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Iimveliso-ibhena

Iimveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA yeKhithi yoVavanyo eFakelwe umfanekiso
  • Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo
  • Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Ikhowudi yeMveliso:


IKHATALOGU

Iinkcukacha zeMveliso

Iithegi zeMveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Isishwankathelo Ukufunyanwa kwezinto ezithileUnyawo kunye noMlomo i-antibodyUhlobo O
Umgaqo

I-FMD Type Asia I antibody ye-ELISA isetyenziselwe ukukhangela izithinteli-ntsholongwane zentsholongwane ye-foot-and-mouth kwiserum yeehagu, iinkomo, iigusha kunye nebhokhwe ukuvavanya ugonyo logonyo lwe-FMD.
Iinjongo zokuFumana Unyawo kunye noMlomo i-antibodyUhlobo O
Isampulu ISerum

 
Ubungakanani Ikhithi e-1 = 192 Uvavanyo
 

 

Uzinzo kunye noGcino

1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce.

2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.

 

 

 

Ulwazi

Intsholongwane yesifo sonyawo kunye nomlomo(FMDV) nguyepathogenoko kubangelaisifo sonyawo nomlomo.[1]Yipicornavirus, ilungu le-prototypical ye-genusAphthovirus.Isifo, esibangela i-vesicles (amadyungudyungu) emlonyeni nasezinyaweni zeiinkomo, iihagu, iigusha, iibhokhwe, nezinyeuphuphu olucandekileyoizilwanyana ziyosulela kakhulu kwaye isibetho esikhuluukulima kwezilwanyana

Umgaqo woVavanyo

Lekitusengokungathanga ngqoELISAmethod,ipuredFMDVantigenispre-coated kwi-enzyme micro-well strips.Xa kuhlolwa, isampulu eyongeziweyo yeserum, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-FMD, iya kudibanisa ne-antigen eqatywe ngaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba;uze ungeze i-enzyme conjugate, ulahle i-enzyme edibeneyo edibeneyo ngokuhlamba.Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso oluhlaza nge-Enzyme catalysis ngumlinganiselo othe ngqo womxholo we-antibody kwisampulu.

Imixholo

 

I-Reagent

Umthamo

Iimvavanyo ezingama-96/192Uvavanyo
1
I-Antigen coated microplate

 

1ea/2ea
2
Ulawulo olubi

 

2ml
3
Ulawulo Olulungileyo

 

1.6ml
4
Iisampulu zokuthambisa

 

100ml
5
Isisombululo sokuhlamba (10X sigxininiswe)

 

100ml
6
I-enzyme conjugate

 

11/22ml
7
Inxalenye engaphantsi

 

11/22ml
8
Ukumisa isisombululo

 

15ml
9
I-Adhesive plate sealer

 

2ea/4ea
10 i-serum dilution microplate

1ea/2ea
11 Umyalelo

iipcs ezi-1

 

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