Iimveliso
Unyawo kunye neSifo soMlomo Uhlobo lwe-Antibody ELISA Test Kit
Unyawo kunye neSifo soMlomo Uhlobo lwe-Antibody ELISA Test Kit
| Isishwankathelo | Ukufunyanwa kwezinto ezithileUhlobo A lwesifo sonyawo kunye nomlomo i-antibody |
| Umgaqo | Ikhithi yovavanyo lwe-FMD yoHlobo lwe-A antibody i-ELISA isetyenziselwa ukufumanisa izithinteli-ntsholongwane zentsholongwane ye-foot-and-mouth kwiserum yeehagu, iinkomo, iigusha kunye neebhokhwe ukuze kuhlolwe ugonyo lwesitofu sokugonya se-FMD. |
| Iinjongo zokuFumana | I-Antibody yesifo sonyawo kunye nomlomo |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
Intsholongwane yesifo sonyawo kunye nomlomo(FMDV) nguyepathogenoko kubangelaisifo sonyawo nomlomo. Yipicornavirus, ilungu le-prototypical ye-genusAphthovirus.Isifo, esibangela i-vesicles (amadyungudyungu) emlonyeni nasezinyaweni zeiinkomo, iihagu, iigusha, iibhokhwe, nezinyeuphuphu olucandekileyoizilwanyana ziyosulela kakhulu kwaye isibetho esikhuluukulima kwezilwanyana.
Umgaqo woVavanyo
Le khithi isebenzisa indlela ye-ELISA ekhuphisanayo kwi-antigens yentsholongwane yesifo sonyawo kunye nomlomo ngaphambili kwimithombo ye-microplate.Xa uvavanya, yongeza isampuli ye-serum ehlanjululweyo kunye ne-anti-FMD ye-Ab, emva kokufukanywa, ukuba kukho i-antibody ye-FMD, iya kudibanisa ne-antigen yangaphambili, i-antibody kwibhloko yesampuli indibaniselwano ye-anti-FMD kunye ne-antigen efakwe ngaphambili;lahla i-enzyme edibeneyo edibeneyo kunye nokuhlamba;Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
IiSerotypes
Intsholongwane yesifo sonyawo kunye nomlomokwenzeka kwisixhenxe esikhuluiiserotypes: O, A, C, SAT-1, SAT-2, SAT-3, kunye ne-Asia-1.Ezi serotypes zibonisa ummandla othile, kwaye i-O serotype ixhaphake kakhulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
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