Iimveliso
Ikhithi yoVavanyo ye-H7 ye-Avian Antibody ELISA
Ikhithi yoVavanyo ye-H7 ye-Avian Antibody ELISA
| Isishwankathelo | I-antibody ye-AIV-H7 isetyenziselwa ikiti yovavanyo ye-ELISA ukukhangela izilwa-buhlungu ze-H7 zoMkhuhlane weAvian kwiserum. |
| Umgaqo | I-antibody ye-AIV-H7 isetyenziselwa ikiti yovavanyo ye-ELISA ukufumanisa i-H7 Subtype Avian Influenza antibodies kwi-serum, ukubeka iliso ku-antibody emva kwe-AIV-H7 yokugonywa komzimba kunye nokuxilongwa kwe-serological yosulelo kwi-Avian. |
| Iinjongo zokuFumana | I-antibody ye-AIV-H7 |
| Isampulu | ISerum
|
| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
|
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
|
Ulwazi
Umkhuhlane weentaka, owaziwa ngokungekho sikweni njengomkhuhlane weentaka okanye umkhuhlane weentaka, ludidi lomkhuhlane olubangelwa ziintsholongwane eziqhelaniswe nazo.iintaka.
Uhlobo olunomngcipheko omkhulu ngumkhuhlane weentaka wentaka (HPAI).Umkhuhlane weentaka uyafana neumkhuhlane wehagu, umkhuhlane wenja, umkhuhlane wehashe kunye
umkhuhlane womntu njengesigulo esibangelwa ziintlobo zentsholongwane zomkhuhlane eziziqhelanise nomkhosi othile.
Kwiintlobo ezintathu zeentsholongwane zomkhuhlane (A,B, kwayeC), intsholongwane yomkhuhlane A yintsholongwane azoonoticusulelo ngedama lendalo phantse
ngokupheleleyo kwiintaka.Umkhuhlane weentaka, ngeenjongo ezininzi, ubhekisa kwintsholongwane yomkhuhlane A.
Umgaqo woVavanyo
Le kit isebenzisa indlela ye-ELISA ekhuphisanayo kwi-antigens ye-AIV-H7 efakwe ngaphambili kwimithombo ye-microplate.Xa uvavanya, yongeza isampulu yeserum ehlanjululweyo kunye ne-enzyme ebhalwe i-anti-AIV-H7 monoclonal antibody, emva kokufukanyelwa, ukuba kukho i-antibody ye-AIV-H7, iya kudibanisa ne-antigen eqatywe ngaphambili, i-antibody kwibloko yesampulu indibaniselwano ye-anti-monoclonal kunye nangaphambili. -i-antigen edibeneyo;lahla i-enzyme edibeneyo edibeneyo kunye nokuhlamba;Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
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