Isishwankathelo | Used ukufumanisa ethile i-antibody ngokuchasene ne-Avian influenza Virus (AIV-H5) kwiserum |
Umgaqo | I-H5 subtype yomkhuhlane weentakai-antibodyIkiti ye-Elisa isetyenziselwa ukufumanisa i-antibody ethileIntaka I-influenza Virus (AIV-H5) kwi-serum, yokubeka iliso kwi-antibody emva kwe-AIV-H5kunye nokuxilongwa kwe-serological yosulelo kwi-Avian.
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Iinjongo zokuFumana | I-antibody yomkhuhlane wentaka i-H5 |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Umkhuhlane weentaka, owaziwa ngokungekho sikweni njengomkhuhlane weentaka okanye umkhuhlane weentaka, ludidi lomkhuhlane olubangelwa ziintsholongwane eziqhelaniswe nazo.iintaka.
Uhlobo olunomngcipheko omkhulu ngumkhuhlane weentaka wentaka (HPAI).Umkhuhlane weentaka uyafana neumkhuhlane wehagu, umkhuhlane wenja, umkhuhlane wehashe kunye
umkhuhlane womntu njengesigulo esibangelwa ziintlobo zentsholongwane zomkhuhlane eziziqhelanise nomkhosi othile.
Kwiintlobo ezintathu zeentsholongwane zomkhuhlane (A,B, kwayeC), intsholongwane yomkhuhlane A yintsholongwane azoonoticusulelo ngedama lendalo phantse
ngokupheleleyo kwiintaka.Umkhuhlane weentaka, ngeenjongo ezininzi, ubhekisa kwintsholongwane yomkhuhlane A.
Le kit isebenzisa ibhloko ye-ELISA indlela, i-antigen ye-AIV-H5 ifakwe ngaphambili kwi-microplate.Xa uvavanya, yongeza isampuli ye-serum ehlanjululweyo, emva kokufakwa, ukuba kukho i-antibody ethile ye-AIV-H5, iya kudibanisa kunye ne-antigen efakwe ngaphambili, ilahle i-antibody engadibaniyo kunye namanye amacandelo ngokuhlamba;uze wongeze i-enzyme ebhalwe i-anti-AIV-H5 i-antibody ye-monoclonal, i-antibody kwibhloko yesampulu indibaniselwano ye-monoclonal antibody kunye ne-antigen eqatywe ngaphambili;lahla i-enzyme engadityaniswanga idibanisa kunye nokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ukwinxalenye eguqukileyo yesiqulatho se-antibody kwisampulu..
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |