Iimveliso-ibhena

Iimveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Ikhowudi yeMveliso:

Igama lento: Unyawo kunye neSifo soMlomo Uhlobo lwe-O Antibody ELISA Test Kit

Isishwankathelo: Isixhobo sovavanyo lwe-FMD Udidi lwe-O lwekiti lwe-ELISA lusetyenziselwa ukubona izilwa-buhlungu zentsholongwane yesifo sonyawo kunye nomlomo kwiserum yeehagu, iinkomo, iigusha kunye nebhokhwe ukuze kuhlolwe ugonyo lwesitofu sokugonya se-FMD.

Iithagethi zokuFumana: I-Antibody ye-O yonyawo kunye nomlomo

Isampulu yoVavanyo: ISerum

Inkcazo: 1 kit = 192 Uvavanyo

Ugcino: Zonke ii-reagents kufuneka zigcinwe kwi-2~8℃.Musa ukuba ngumkhenkce.

Ixesha leShelf: iinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Isishwankathelo Ukufunyanwa kwezinto ezithileUnyawo kunye noMlomo i-antibodyUhlobo O
Umgaqo

I-FMD Type Asia I antibody ye-ELISA isetyenziselwe ukukhangela izilwa-buhlungu zentsholongwane yesifo sonyawo kunye nomlomo kwiserum yeehagu, iinkomo, iigusha kunye neebhokhwe ukuze kuhlolwe ugonyo lwesitofu sogonyo lwe-FMD.

Iinjongo zokuFumana Unyawo kunye noMlomo i-antibodyUhlobo O
Isampulu ISerum

 

Ubungakanani Ikhithi e-1 = 192 Uvavanyo
 

 

Uzinzo kunye noGcino

1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce.

2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.

 

 

 

Ulwazi

Intsholongwane yesifo sonyawo kunye nomlomo(FMDV) nguyepathogenoko kubangelaisifo sonyawo nomlomo.[1]Yipicornavirus, ilungu le-prototypical ye-genusAphthovirus.Isifo, esibangela i-vesicles (amadyungudyungu) emlonyeni nasezinyaweni zeiinkomo, iihagu, iigusha, iibhokhwe, nezinyeuphuphu olucandekileyoizilwanyana ziyosulela kakhulu kwaye isibetho esikhuluukulima kwezilwanyana

Umgaqo woVavanyo

Lekitusengokungathanga ngqoELISAndlela,ipuredFMDVantigenispre-coated kwi-enzyme micro-well strips.Xa kuhlolwa, isampulu ye-serum eyongeziweyo, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-FMD, iya kudibanisa ne-antigen eqatywe ngaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba;uze ungeze i-enzyme conjugate, ulahle i-enzyme edibeneyo edibeneyo ngokuhlamba.Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso oluhlaza nge-Enzyme catalysis ngumlinganiselo othe ngqo womxholo we-antibody kwisampulu.

Imixholo

 

I-Reagent

Umthamo

Iimvavanyo ezingama-96/192Uvavanyo

1
I-Antigen coated microplate

 

1ea/2ea

2
Ulawulo olubi

 

2ml

3
Ulawulo Olulungileyo

 

1.6ml

4
Iisampulu zokuthambisa

 

100ml

5
Isisombululo sokuhlamba (10X sigxininiswe)

 

100ml

6
I-enzyme conjugate

 

11/22ml

7
Inxalenye engaphantsi

 

11/22ml

8
Ukumisa isisombululo

 

15ml

9
I-Adhesive plate sealer

 

2ea/4ea

10 i-serum dilution microplate

1ea/2ea

11 Umyalelo

iipcs ezi-1

 


  • Ngaphambili:
  • Okulandelayo:

  • Bhala umyalezo wakho apha kwaye uwuthumele kuthi