Isishwankathelo | Ukufunyanwa kwezinto ezithileUnyawo kunye noMlomo i-antibodyUhlobo O |
Umgaqo | I-FMD Type Asia I antibody ye-ELISA isetyenziselwe ukukhangela izilwa-buhlungu zentsholongwane yesifo sonyawo kunye nomlomo kwiserum yeehagu, iinkomo, iigusha kunye neebhokhwe ukuze kuhlolwe ugonyo lwesitofu sogonyo lwe-FMD. |
Iinjongo zokuFumana | Unyawo kunye noMlomo i-antibodyUhlobo O |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Intsholongwane yesifo sonyawo kunye nomlomo(FMDV) nguyepathogenoko kubangelaisifo sonyawo nomlomo.[1]Yipicornavirus, ilungu le-prototypical ye-genusAphthovirus.Isifo, esibangela i-vesicles (amadyungudyungu) emlonyeni nasezinyaweni zeiinkomo, iihagu, iigusha, iibhokhwe, nezinyeuphuphu olucandekileyoizilwanyana ziyosulela kakhulu kwaye isibetho esikhuluukulima kwezilwanyana
Lekitusengokungathanga ngqoELISAndlela,ipuredFMDVantigenispre-coated kwi-enzyme micro-well strips.Xa kuhlolwa, isampulu ye-serum eyongeziweyo, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-FMD, iya kudibanisa ne-antigen eqatywe ngaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba;uze ungeze i-enzyme conjugate, ulahle i-enzyme edibeneyo edibeneyo ngokuhlamba.Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso oluhlaza nge-Enzyme catalysis ngumlinganiselo othe ngqo womxholo we-antibody kwisampulu.
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |