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Iimveliso-ibhena

Iimveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA yeKhithi yoVavanyo eFakelwe umfanekiso
  • Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo
  • Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Ikhowudi yeMveliso:

Igama lento: Unyawo kunye neSifo soMlomo Uhlobo lwe-O Antibody ELISA Test Kit

Isishwankathelo: Isixhobo sovavanyo lwe-FMD Udidi lwe-O lwekiti lwe-ELISA lusetyenziselwa ukubona izilwa-buhlungu zentsholongwane yesifo sonyawo kunye nomlomo kwiserum yeehagu, iinkomo, iigusha kunye nebhokhwe ukuze kuhlolwe ugonyo lwesitofu sokugonya se-FMD.

Iithagethi zokuFumana: I-Antibody ye-O yonyawo kunye nomlomo

Isampulu yoVavanyo: ISerum

Inkcazo: 1 kit = 192 Uvavanyo

Ugcino: Zonke ii-reagents kufuneka zigcinwe kwi-2~8℃. Musa ukuba ngumkhenkce.

Ixesha leShelf: iinyanga ezili-12. Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.


IKHATALOGU

Iinkcukacha zeMveliso

Iithegi zeMveliso

Unyawo kunye neSifo soMlomo Uhlobo lwe-O Ab ELISA Ikhithi yoVavanyo

Isishwankathelo Ukufunyanwa kwezinto ezithileUnyawo kunye noMlomo i-antibodyUhlobo O
Umgaqo

I-FMD Type Asia I antibody ye-ELISA isetyenziselwe ukukhangela izilwa-buhlungu zentsholongwane yesifo sonyawo kunye nomlomo kwiserum yeehagu, iinkomo, iigusha kunye neebhokhwe ukuze kuhlolwe ugonyo lwesitofu sogonyo lwe-FMD.
Iinjongo zokuFumana Unyawo kunye noMlomo i-antibodyUhlobo O
Isampulu ISerum

 
Ubungakanani Ikhithi e-1 = 192 Uvavanyo
 

 

Uzinzo kunye noGcino

1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃. Musa ukuba ngumkhenkce.

2) Ubomi beShelf ziinyanga ezili-12. Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.

 

 

 

Ulwazi

Intsholongwane yesifo sonyawo kunye nomlomo(FMDV) nguyepathogenoko kubangelaisifo sonyawo nomlomo.[1]Yipicornavirus, ilungu le-prototypical ye-genusAphthovirus. Isifo, esibangela i-vesicles (amadyungudyungu) emlonyeni nasezinyaweni zeiinkomo, iihagu, iigusha, iibhokhwe, nezinyeuphuphu olucandekileyoizilwanyana ziyosulela kakhulu kwaye isibetho esikhuluukulima kwezilwanyana

Umgaqo woVavanyo

Lekitusengokungathanga ngqoELISAmethod,ipuredFMDVantigenispre-coated kwi-enzyme micro-well strips. Xa kuhlolwa, isampulu eyongeziweyo yeserum, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-FMD, iya kudibanisa ne-antigen eqatywe ngaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba; uze ungeze i-enzyme conjugate, ulahle i-enzyme edibeneyo edibeneyo ngokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso oluhlaza nge-Enzyme catalysis ngumlinganiselo othe ngqo womxholo we-antibody kwisampulu.

Imixholo

 

I-Reagent

Umthamo

Iimvavanyo ezingama-96/192Uvavanyo
1
I-Antigen coated microplate

 

1ea/2ea
2
Ulawulo olubi

 

2ml
3
Ulawulo Olulungileyo

 

1.6ml
4
Iisampulu zokuthambisa

 

100ml
5
Isisombululo sokuhlamba (10X sigxininiswe)

 

100ml
6
I-enzyme conjugate

 

11/22ml
7
ISubstrate

 

11/22ml
8
Ukumisa isisombululo

 

15ml
9
I-Adhesive plate sealer

 

2ea/4ea
10 i-serum dilution microplate

1ea/2ea
11 Umyalelo

iipcs ezi-1

 

  • Ngaphambili:
  • Okulandelayo:
    • Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    iimveliso ezinxulumene

    Cofa u-enter ukukhangela okanye i-ESC ukuvala