Iimveliso
IAvian Leukemia P27 Antigen ELISA Kit
I-Hydatid Disease Antibody ELISA Kit
| Isishwankathelo | isetyenziselwa ukubhaqa i-Avian leukosis P27 antigen kwigazi le-avian, ilindle, i-cloaca, kunye neqanda elimhlophe. |
| Umgaqo | I-Avian Leukosis (AL) Ikhithi ye-antigen ye-P27 ye-Elisa isetyenziselwa ukuqaphela i-antigen ye-Avian leukosis P27 egazini lentaka, ilindle, i-cloaca, kunye neqanda elimhlophe.
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| Iinjongo zokuFumana | I-Avian Leukosis (AL) i-antigen ye-P27 |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃. Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12. Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
I-Avian Leukosis (AL) ligama elidityanisiweyo lezifo ezahlukeneyo ezinxulumene nethumba kwiinkukhu ezibangelwa yi-Avian Leukosis Virus (ALV) kusapho lweRetroviridae. Esi sifo sisasazwa kwihlabathi jikelele kwaye sinezinga eliphezulu losulelo. Ingadala ukufa kunye nokubhitya kwenkukhu, yehlise umthamo wemveliso yomhlambi, kwaye sesinye sezifo eziphambili ezibeka emngciphekweni uphuhliso loshishino lweenkukhu. Esi sifo sinembali ende kwaye sihlala sijongene neemeko ezintsha, ezifana ne-Avian leukemia virus subgroup J (ALV-J), eyafunyanwa yaza yachongwa ekupheleni kweminyaka yoo-1980 e-UK njenge-subtype entsha ye-avian leukemia virus, eyenza umonakalo omkhulu kwishishini le-broiler.
Umgaqo woVavanyo
Ikiti isebenzisa indlela ye-sandwich ELISA, i-pured anti-avian leukocyte i-P27 i-monoclonal antibody ifakwe ngaphambili kwi-enzyme micro-well strips.Kuvavanyo, i-antigen kwisampulu ibophelelwe kwi-antibody kwi-plate edibeneyo, emva kokuhlamba ukususa i-antigen engabonakaliyo kunye nezinye izinto, i-enzyme ye-monoclonal antibody yongezwa kwi-antigen ye-antigen eyinkimbinkimbi yongezwa kwi-antigen eyinkimbinkimbi kwi-antigen. emva koko ukuhlamba, i-enzyme conjugate engabotshwanga isusiwe, isisombululo se-TMB substrate songezwa kwi-microplate, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis iyingxenye ngokuthe ngqo yomxholo we-antibody kwisampuli. Yongeza isisombululo sokumisa, Emva kokusabela, i-absorbeance Ixabiso kwi-reaction well ilinganiswa ne-wavelength ye-450 nm.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
