Isishwankathelo | Isetyenziselwa ukufumanisa izilwa-buhlungu ezithile ezichasene ne-avian influenza virus (AIV) kwiserum |
Umgaqo | Ikhithi ye-Avian influenza antibody Elisa isetyenziselwa ukufumanisa i-antibody ethile echasene ne-Avian influenza Virus (AIV) kwiserum, ukujonga i-antibody emva kwe-AIV yokugonywa kunye nokuxilongwa kwe-serological yosulelo kwi-Avian.. |
Iinjongo zokuFumana | I-Avian Influenza Antibody |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Umkhuhlane weentaka, owaziwa ngokungekho sikweni njengomkhuhlane weentaka okanye umkhuhlane weentaka, ludidi lomkhuhlane olubangelwa ziintsholongwane eziqhelaniswe nazo.iintaka.
Uhlobo olunomngcipheko omkhulu ngumkhuhlane weentaka wentaka (HPAI).Umkhuhlane weentaka uyafana neumkhuhlane wehagu, umkhuhlane wenja, ihashe
umkhuhlane kunye nomkhuhlane womntu njengesigulo esibangelwa ziintlobo zentsholongwane zomkhuhlane ezithe zaqhelana nenye
umamkeli.Kwiintlobo ezintathu zeentsholongwane zomkhuhlane (A,B, kwayeC), intsholongwane yomkhuhlane A yintsholongwane azoonoticusulelo ngendalo
indawo yokugcina amanzi phantse ngokupheleleyo kwiintaka.Umkhuhlane weentaka, ngeenjongo ezininzi, ubhekisa kwintsholongwane yomkhuhlane A.
Le kit isebenzisa ibhloko indlela ye-ELISA, i-antigen ye-AIV ifakwe ngaphambili kwi-microplate.Xa uvavanya, yongeza isampulu ye-serum ehlanjululweyo, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-AIV, iya kudibanisa ne-antigen yangaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba;emva koko wongeze i-enzyme ebhalwe i-anti-AIV monoclonal antibody, i-antibody kwibloko yesampuli indibaniselwano ye-monoclonal antibody kunye ne-antigen eqatywe ngaphambili;lahla i-enzyme engadityaniswanga idibanisa kunye nokuhlamba.Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |