Brucella Ag Test Kit | |
Inombolo yekhathalogu | I-RC-CF10 |
Isishwankathelo | Ukufunyanwa kwe-antigen ethile ye-Brucella ngaphakathi kwemizuzu eyi-10 |
Umgaqo | Inyathelo elinye lovavanyo lwe-immunochromatographic |
Iinjongo zokuFumana | Brucella antigen |
Isampulu | I-Canine, i-bovine kunye ne-Ovis Igazi elipheleleyo, i-Plasma okanye iSerum |
Ixesha lokufunda | 10 ~ 15 imizuzu |
Uvakalelo | 91.3 % vs. IFA |
Ukuchaza ngokuthe ngqo | 100.0 % vs. IFA |
Umda wokuFumana | IFA Titer 1/16 |
Ubungakanani | Ibhokisi ye-1 (ikhithi) = izixhobo ezili-10 (Ukupakishwa komntu ngamnye) |
Imixholo | Ikhithi yovavanyo, iiThubhu, iidropha ezilahlwayo |
Isilumkiso | Sebenzisa phakathi kwemizuzu eyi-10 emva kokuvula Sebenzisa isixa esifanelekileyo sesampulu (0.01 ml yedropha) Sebenzisa emva kwe-15 ~ 30 imizuzu kwi-RT ukuba zigcinwe phantsi kweemeko ezibandayo Thatha iziphumo zovavanyo njengezingasebenziyo emva kwemizuzu eli-10 |
I-genus Brucella lilungu losapho lweBrucellaceae kwaye ibandakanya iindidi ezilishumi ezincinci, ezingenamoyile, ezingenasporing, aerobic, gram-negative intracellular coccobacilli.Ziyi-catalase, i-oxidase kunye ne-urea positive bacteria.Amalungu ohlobo angakhula kwimidiya ephuculweyo njenge-agar yegazi okanye i-chocolate agar.I-Brucellosis yi-zoonosis eyaziwayo, ekhoyo kuwo onke amazwekazi, kodwa ngokuxhaphaka kakhulu kunye neziganeko, kwizilwanyana kunye nabantu.I-Brucella, njenge-facultative intracellular parasites, i-colonize iintlobo ezininzi zezilwanyana zentlalo ngendlela engapheliyo, enokwenzeka ngokusisigxina, mhlawumbi ubomi babo bonke.
Ikoloni yaseBrucella inkangeleko
Iintlobo zeBrucella zikholisa ukosulela phakathi kwezilwanyana ngokudibana neplacenta, umbungu, ulwelo olungekazalwa kunye nokuphuma kwilungu lobufazi.isilwanyana esosulelekileyo.Uninzi okanye zonke iintlobo zeBrucella nazo ziyafumaneka kwisemen.Inkunzi inokuchitha ezi ntsholongwane ixesha elide okanye ubomi bonke.Ezinye iintlobo zeBrucella ziye zabhaqwa kwezinye izinto ezikhutshwayo eziquka umchamo, ilindle, i-hygroma fluid, isalvia, ubisi kunye nencindi yeempumlo kunye ne-ocular.
I-Ecology yosulelo lwe-zoonotic Brucella
Ezinkomeni
Akukho ndlela isebenzayo yokubona izilwanyana ezosulelekileyo ngenkangeleko yazo.Ezona mpawu zicacileyo kwizilwanyana ezimithiyo kukuphunza okanye ukuzalwa kwamathole abuthathaka.Ukuveliswa kobisi kunokuncitshiswa kwiinguqu kwixesha eliqhelekileyo lokuncancisa okubangelwa kukuphunza kunye nokukhawula okulibaziseka.Ezinye iimpawu ze-brucellosis ziquka ukuhla okubonakalayo kokuzala kunye namazinga aphantsi okukhulelwa, ukugcinwa kokubeleka emva kokuzalwa okubangelwa usulelo lwesibeleko, kunye (ngamathuba athile) kunye nokwandiswa kwamalungu e-arthritis.
Ezinjeni
Ezinjeni, ibhaktheriya ye-Brucellosis idla ngokuhlala phantsi kumalungu angasese kunye ne-lymphatic system, kodwa kunokwenzeka ukuba isasazeke kwizintso, amehlo kunye ne-intervertebral disc ngokunjalo.Xa i-Brucellosis ichaphazela i-disc intervertebral, umphumo we-discopondylitis.Kwizinja, iimpawu ezivela kwizitho zokuzala ziqhelekileyo.Izinja ezingamadoda zinokwenza umzekelo ukudumba kwe-scrotal kunye ne-testicular, ngelixa izinja ezizimazi zinokuphunyelwa zizisu.Umkhuhlane awuqhelekanga, kodwa iintlungu ezinxulumene neBrucellosis zinokwenza inja ibe buthathaka.Ukuba isifo sisasazeka kwizintso, amehlo okanye iimpawu ze-disc intervertebral zingaqala ukubonisa kula malungu.
Kwiihagu
Ixesha phakathi kokusuleleka kunye nokubonakala kweempawu zeklinikhi zesifo kunokuvela malunga neveki ye-1 ukuya kwiinyanga ezi-2.Iimpawu zokuba umhlambi wosulelekile ikakhulu zezo zokusilela ekuzaleni - ukuphunza, ukubuyela enkonzweni emva kokukhwelana kunye nokuzala kwamantshontsho abuthathaka okanye azelwe sele eswelekile.Ezinye iimazi zinokuvela usulelo lwesibeleko kwaye zibonise ukuphuma kwilungu lobufazi.Iinkunzi zeehagu ezosulelekileyo zinokudumba, amasende adumbe.Zombini izini zinokuqhwalela kunye namalungu adumbileyo kunye/okanye zibe neempawu zokungalungelelani kunye nokukhubazeka kwemilenze yangasemva.
1.Ukwahlukaniswa kunye nokuchongwa kwe-arhente
Iintlobo ze-Brucella zinokufunyanwa kwiithishu ezininzi kunye nokuphuma, ngakumbi inwebu yomntwana, ukukhutshwa kwelungu lobufazi, ubisi (okanye ukukhutshwa kombele), amadlozi, isifo samathambo se-hygroma fluids, kunye nesiqulatho sesisu, udakada nemiphunga kwiimveku eziqhomfayo.Uninzi lweentlobo zeBrucella ezivela kwiikholoni kwiintsuku ezimbalwa kwiimidiya ezikhethiweyo.Xa iipleyiti zijongwa emini nge-transparent medium, ezi koloni ziguquguqukayo kunye nombala wobusi obomvu.Xa ujongwe phezulu, amakoloni abonakala econvex kwaye amhlophe njengeperile.Emva koko iikoloni ziba nkulu kwaye zibe mnyama kancinci.
2.Nucleic acid Indlela
I-PCR sisixhobo esifanelekileyo sokuxilongwa kwe-brucellosis.Uvavanyo oluninzi olusekwe kwi-PCR luphuhliselwe ukuchongwa kweBrucella ukuphucula ukukwazi ukuxilonga.Uvavanyo lwe-PCR olukhethekileyo lufanelekile ekuchongeni okulula kukaBrucella.
3.Ukuxilongwa kwe-serological
Kukho iimvavanyo ezininzi ze-serological.Uvavanyo lwe-serological oluqhele ukusetyenziswa ukuvavanya iinkomo okanye umhlambi ngamnye lubandakanya uvavanyo lwe-antigen ye-Brucella, ukulungiswa kokuncedisana, i-enzyme-linked immunosorbent assays (ELISA) kunye novavanyo lwe-fluorescence.