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Itekhnoloji yokufumanisa i-Enzvme ye-Enterococcus yovavanyo lwamanzi oMfanekiso oFakelweyo
  • Itekhnoloji yokufumanisa i-Enzvme ye-Enterococcus Kuvavanyo lwamanzi
  • Itekhnoloji yokufumanisa i-Enzvme ye-Enterococcus Kuvavanyo lwamanzi

Itekhnoloji yokufumanisa i-Enzvme ye-Enterococcus Kuvavanyo lwamanzi

Ikhowudi yeMveliso:

Igama lenqaku:Itekhnoloji yokufumanisa i-Enzvme ye-Enterococcu

Umlingiswa: Le mveliso imhlophe okanye ilula amasuntswana atyheli Ukucaca

Ezingenambala:okanye ezityheli ezikhanyayo

pH 7.0 - 7.6

Ubunzima: 2.7 士 0.5g

Ugcino: Ukugcinwa kwi-4°C – 8°C, kwindawo epholileyo eyomileyo kwaye ukhusele ekukhanyeni

Ukunyaniseka: Unyaka we-1, Bona ukupakishwa kwe-reagent yomhla wemveliso kunye nomhla wokuphelelwa kwexesha.

Inzululwazi:Yongeza isampulu yamanzi equlethe ibhaktheriya ye-Enterococcus, inkcubeko yebhaktheriya ekujoliswe kuyo kwi-Mug medium kwi-41 ° C ukuya ku-0.5 ° C, kunye ne-enzyme ethile ye-ological eveliswa yi-Enterococcus bacteria (3 -0 -gluco sidase inokubola

i-fluorescent substrate imagi kwi-magi medium ukuvelisa (3 -D-glucoside ((3 -0 -glucoside) kunye

uphawu lwemveliso ye-fluorescent 4-methyl umbelferone.Qwalasela i-fluorescence kwi-366nm isibane se-UV, ubale ngediski yokufumanisa ubungakanani, kwaye ubuze itafile yeMPN ukubala iziphumo.

Iphakheji: 100 - ipakethe yokuvavanya


IKHATALOGU

Iinkcukacha zeMveliso

Iithegi zeMveliso

asdasd1
asdasd2
asd3

Umlinganiswa

 Le mveliso inamasuntswana amhlophe okanye atyheli akhanyayo

Ukucaca

 Ayinambala okanye ityheli ekhanyayo

PH

 7.0 - 7.6

WSibhozo

 2.7 士 0.5g

Ugcino

 kwi-4°C - 8°C, kwindawo epholileyo eyomileyo kwaye ukhusele ekukhanyeni

Ukunyaniseka

 1 Unyaka, Bona ukupakishwa kwe-reagent yomhla wemveliso kunye nomhla wokuphelelwa.

Inzululwazi

 Yongeza isampuli yamanzi equlethe ibhaktheriya ye-Enterococcus, inkcubeko ibhaktheriya ekujoliswe kuyo kwi-Mug medium kwi-41 ° C 土 0.5 ° C, kunye ne-enzyme ethile ye-biological eveliswa yi-Enterococcus bacteria (β-D-glucosidase inokubola i-fluorescent substrate mug kwi-mag medium ukuvelisa. (β-D-glucoside ((β-D-glucoside) kunye nemveliso ye-fluorescent ye-4-methyl umbelliferone. Qaphela i-fluorescence kwisibane se-UV ye-366nm, ubale nge-disk yokufumanisa ubungakanani, kwaye ubuze itafile ye-MPN ukubala iziphumo.

Iphakheji

 I-100 - ipakethe yokuvavanya

Indlela yokusetyenziswa Uvavanyo lomgangatho

I-Setp1:Thatha i-100ml yesampuli yamanzi kunye ne-100ml ibhotile yesampula engenazintsholongwane / ibhotile yobungakanani, yongeza i-reagent, i-dissolve, kunye nenkcubeko ye-24h kwi-41 ° C 士 0 .5°C.

I-Setp2: Isigwebo sesiphumo

I-Non fluorescence = Iziphumo ezibi Ifluorescence eluhlaza okwesibhakabhaka = Iziphumo ezilungileyo

Ukufumanisa ubungakanani

1.Sebenzisa i-100ml ibhotile yesampuli engasebenziyo ukulinganisa i-100ml yesampuli yamanzi.

2.Yongeza i-reagent, yinyibilikise, yigalele kwi-51 Quanti Tray okanye 97 Quanti-Tray.

3.Pakisha iQuanti-Tray ngomatshini olawulwayo wokutywina wobungakanani, kwaye uyifukamele kwi-41°C 士 0 .5 ° C nge-24h.

4.Jonga kuvavanyo lomgangatho wokutolikwa kweziphumo, kwaye ujonge itafile yeMPN ukubala.

  • Ngaphambili:
  • Okulandelayo:
    • Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    iimveliso ezinxulumene

    Cofa u-enter ukukhangela okanye i-ESC ukuvala