Isishwankathelo | Ukufunyanwa kwe-Antibody isifo se-Hydatid |
Umgaqo | Ikhithi yovavanyo lwe-Hydatid ye-antibody ye-Elisa inokusetyenziselwa ukubona i-antibody ye-Hydatid kwi-serum yeenkomo, iibhokhwe kunye neegusha. |
Iinjongo zokuFumana | I-antibody ye-Hydatid |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ikwabizwa ngokuba sisifo se-hydatid, sisifo se-parasitic esinokuchaphazela abantu kunye nezinye izilwanyana ezincancisayo ezifana neegusha, izinja, iimpuku kunye namahashe.Iintlobo ezintathu ezahlukeneyo ze-echinococcosis zifumaneka ebantwini, nganye ibangelwa yimibungu yeentlobo ezahlukeneyo ze-Echinococcus granulosus tapeworm.Isifo sokuqala esafunyanwa ebantwini yi-cystic echinococcosis (ekwaziwa ngokuba yi-cystic echinococcosis), ebangelwa ngu-Echinococcus granulosus (igama lesayensi: Echinococcus granulosus).Indawo yesibini yi-alveolar echinococcosis (eyaziwa ngokuba yi-alveolar echinococcosis), ebangelwa yi-follicular echinococcosis (igama lesayensi: Echinococcus multilocularis).Emva kokuqala, iimpawu zesigulane kunye neempawu zixhomekeke kwindawo kunye nobukhulu be-echinococcosis.I-Alveolar echinococcosis ihlala iqala esibindini, kodwa inokusasazeka kwezinye iindawo, njengemiphunga kunye nengqondo.Emva kokuvela kwezilonda zesibindi, iimpawu zekliniki zezigulane zinokubandakanya intlungu yesisu, ukuncipha komzimba, kunye ne-jaundice.Izilonda emiphungeni ezinokubangela intlungu esifubeni, ukuphefumla nzima, kunye nokukhohlela
Oku ikhithi sebenzisa ngokungathanga ngqo ELISA indlela, ihlanjululwe I-antigen ye-HYD is iqatywe ngaphambili on i-enzyme micro kakuhle imicu. Xa uvavanya, yongeza ixutywe serum isampuli, emva incubation, if Pha is I-HYD intsholongwane ethile amajoni omzimba, it intando dibanisa nge i iqatywe ngaphambili i-antigen, lahla i engadityaniswanga i-antibody kwaye enye amacandelo nge ukuhlamba; ngoko yongeza i-enzyme dibanisa, lahla i engadityaniswanga i-enzyme dibanisa ngokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ohlaza okwesibhakabhaka nge-Enzyme catalysis ngokuthe ngqo Umlinganiselo wesiqulatho se-antibody kwisampulu.
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |