Iimveliso
Bovine Tuberculosis Antibody ELISA Kit
Bovine Tuberculosis Antibody ELISA Kit
| Isishwankathelo | Ukufunyanwa kwe-antibody ethile ye-Bovine Tuberculosis(BTB). |
| Umgaqo | Ikiti yovavanyo lwe-Bovine Tuberculosis(BTB) i-Elisa inokusetyenziselwa ukubona i-antibody ye-Bovine Tuberculosis kwiserum okanye kwiplasma yeBovine. |
| Iinjongo zokuFumana | I-antibody ye-Bovine Tuberculosis(BTB). |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
I-Mycobacterium bovis yintsholongwane ekhula kade (i-16- ukuya kwiiyure ezingama-20 kwixesha lesizukulwana) ibhaktiriya ene-aerobic kunye ne-causative agent yesifo sephepha ezinkomeni (eyaziwa ngokuba yi-bovine TB).Inxulumene ne-Mycobacterium tuberculosis, ibhaktiriya ebangela isifo sephepha ebantwini.I-M. bovis inokutsiba umqobo wohlobo kwaye ibangele usulelo olufana nesifo sephepha ebantwini nakwezinye izilwanyana ezanyisayo.
Umgaqo woVavanyo
Oku ikhithi sebenzisa ngokungathanga ngqo ELISA indlela, ihlanjululwe I-BTB i-antigen is iqatywe ngaphambili on i-enzyme micro kakuhle imicu. Xa uvavanya, yongeza ixutywe serum isampuli, emva incubation, if Pha is I-BTB ethile amajoni omzimba, it intando dibanisa nge i iqatywe ngaphambili i-antigen, lahla i engadityaniswanga i-antibody kwaye enye amacandelo nge ukuhlamba; ngoko yongeza i-enzyme dibanisa, lahla i engadityaniswanga i-enzyme dibanisa nge
ukuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ohlaza okwesibhakabhaka nge-Enzyme catalysis ngokuthe ngqo Umlinganiselo wesiqulatho se-antibody kwisampulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
