Iimveliso
Peste Des Petits Ruminants Ab ELISA Kit
Peste Des Petits Ruminants Ab ELISA Kit
| Isishwankathelo | Ukufunyanwa kweAntibody ethile yePeste Des Petits Ruminants |
| Umgaqo | Izixhobo zovavanyo ze-PPRV ze-ELISA zisetyenziselwa ukubona izilwa-buhlungu zentsholongwane zePeste des petits ruminants kwiserum yeegusha neebhokhwe. |
| Iinjongo zokuFumana | I-antibody ye-PPRV |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
I-Ovine rinderpest, eyaziwa ngokuba yipeste des petits ezetyisayo(PPR), sisifo esosulelayo esichaphazela ikakhuluiibhokhwekwayeiigusha;nangona kunjalo, iinkamela kunye nezilwanyana ezincinciezetyisayoinokuchaphazeleka.I-PPR okwangoku ikhonaMantla, Umbindi, eNtshonakwayeEMpuma Afrika, iPhakathi empuma, kwayeEMzantsi Asia. Ibangelwaiiruminants ezincinci morbilliviruskwi-genusI-Morbillivirus,kwaye inxulumene ngokusondeleyo, phakathi kwezinye, i-rinderpest morbillivirus, imasisi morbillivirus, kwayei-canine morbillivirus(eyayisaziwa njengeinkaniintsholongwane ye-distemper).Esi sifo sosulela kakhulu, kwaye sinokuba nesantya sokusweleka esingama-80-100%.ebukhaliiimeko kwiepizooticulungiselelo.Le ntsholongwane ayibasuleli abantu.
I-PPR ikwabizwa ngokuba sisibetho seebhokhwe.kata, i-syndrome ye-stomatitis-pneumoenteritis, kunye ne-ovine rinderpest.
Ii-arhente ezisemthethweni ezifana neI-FAOkwayeOIEsebenzisa igama lesiFrentshi"peste des petits ezetyisayo" ngeendlela ezininzi zopelo.
Umgaqo woVavanyo
Le khithi isebenzisa indlela ye-ELISA ekhuphisanayo kwi-antigens ye-PPRV efakwe ngaphambili kwimithombo ye-microplate.Xa uvavanya, yongeza isampulu ye-serum ehlanjululweyo, emva kokufukanyelwa, ukuba kukho i-antibody ye-PPRV, iya kudibanisa ne-antigen eqatywe ngaphambili, i-antibody kwibhloko yesampulu indibaniselwano ye-monoclonal antibody kunye ne-antigen efakwe ngaphambili;lahla i-enzyme edibeneyo edibeneyo kunye nokuhlamba;Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
