Iimveliso
Newcastle Disease Antibody ELISA Kit
Newcastle Disease Antibody ELISA Kit
| Isishwankathelo | Ukufunyanwa kwe-Antibody ethile ye-Newcastle antibodies ezithile |
| Umgaqo | Ikhithi ye-antibody yeNewcastle Elisa isetyenziselwa ukukhangela amajoni athile omzimba achasene nesifo saseNewcastle Intsholongwane (NDV) kwiserum, yokuhlola i-antibody emva kwe-NDV immunekunye nokuxilongwa kwe-serological yosulelo kwi-Avian.
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| Iinjongo zokuFumana | I-antibody ye-Newcastle |
| Isampulu | ISerum
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| Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
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Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Ulwazi
Isifo saseNewcastle sisifo seentaka esisulelayo esichaphazela iintlobo ezininzi zeentaka zasekhaya kunye nezasendle;iyosulela ebantwini.Nangona inokosulela abantu, iimeko ezininzi azinazimpawu;kunqabile ukuba kubangele umkhuhlane ongephi kunye neempawu ezifana nomkhuhlane kunye/okanye ujunctivitis ebantwini.Iziphumo zayo ziphawuleka kakhulu kwiinkukhu zasekhaya ngenxa yokuba semngciphekweni okuphezulu kunye nokubanakho kweempembelelo ezimandundu ze-epizootic kwimizi-mveliso yeenkukhu.Ixhaphakile kumazwe amaninzi.Akukho lunyango lwalo lwaziwayo, kodwa ukusetyenziswa kwezitofu zokugonya kunye nemilinganiselo yococeko kunciphisa ukubakho koqhambuko.
Umgaqo woVavanyo
Le kit isebenzisa ibhloko indlela ye-ELISA, i-antigen ye-NDV ifakwe ngaphambili kwi-microplate.Xa uvavanya, yongeza isampulu ye-serum ehlanjululweyo, emva kokufukanyelwa, ukuba kukho i-antibody ethile ye-NDV, iya kudibanisa ne-antigen yangaphambili, ilahle i-antibody engadityaniswanga kunye namanye amacandelo ngokuhlamba;emva koko wongeze i-enzyme ebhalwe i-anti-NDV monoclonal antibody, i-antibody kwibhloko yesampulu indibaniselwano ye-monoclonal antibody kunye ne-antigen eqatywe ngaphambili;lahla i-enzyme engadityaniswanga idibanisa kunye nokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ikwi-inverse proportion ye-antibody content kwisampulu.
Imixholo
| I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | i-serum dilution microplate | 1ea/2ea | |
| 11 | Umyalelo | iipcs ezi-1 |
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