Inombolo yekhathalogu | I-RC-CF28 |
Isishwankathelo | Ukufunyanwa kwe-anti-Toxoplasma IgG/IgM antibodies ngaphakathi kwemizuzu eyi-10 |
Umgaqo | Inyathelo elinye lovavanyo lwe-immunochromatographic |
Iinjongo zokuFumana | I-antibody ye-Toxoplasma IgG/IgM |
Isampulu | Igazi elipheleleyo leFeline, iPlasma okanye iSerum |
Ixesha lokufunda | 10 ~ 15 imizuzu |
Uvakalelo | IgG : 97.0 % vs. IFA , IgM : 100.0 % vs. IFA |
Ukuchaza ngokuthe ngqo | IgG : 96.0 % vs. IFA , IgM : 98.0 % vs. IFA |
Ubungakanani | Ibhokisi ye-1 (ikhithi) = izixhobo ezili-10 (Ukupakishwa komntu ngamnye) |
Imixholo | Ikhithi yovavanyo, ibhotile yeBuffer, kunye needropha eziLahlwayo |
Ugcino | Ubushushu beGumbi (ku-2 ~ 30℃) |
Ukuphelelwa lixesha | Iinyanga ezingama-24 emva kokuveliswa |
Isilumkiso | Sebenzisa phakathi kwemizuzu eyi-10 emva kokuvulaSebenzisa isixa esifanelekileyo sesampulu (0.01 ml yedropha) Sebenzisa emva kwe-15 ~ 30 imizuzu kwi-RT ukuba zigcinwe phantsi kweemeko ezibandayo Thatha iziphumo zovavanyo njengezingasebenziyo emva kwemizuzu eli-10 |
I-Toxoplasmosis sisifo esibangelwa sisifunxi-gazi esineseli enye ebizwa ngokuba yiToxoplasma gondii (T.gondii).I-Toxoplasmosis sesinye sezona zifo zixhaphakileyo ze-parasitic kwaye ifunyenwe phantse kuzo zonke izilwanyana ezinegazi elifudumeleyo, kubandakanya izilwanyana zasekhaya kunye nabantu.Iikati zibalulekile kwi-epidemiology ye-T. gondii kuba kuphela kwemikhosi ekwazi ukukhupha ii-oocysts ezimelana nokusingqongileyo.Uninzi lweekati ezosulelwe yi-T.gondii aziyi kubonakalisa naziphi na iimpawu.Ngamanye amaxesha, nangona kunjalo, isifo seklinikhi se-toxoplasmosis siyenzeka.Xa isifo senzeke, sinokukhula xa impendulo ye-immune yekati ayikwanelanga ukumisa ukusasazeka kweefom ze-tachyzoite.Esi sifo sinokwenzeka ukuba senzeke kwiikati ezinama-immune systems, kubandakanywa amakati amancinci kunye neekati ezine-feline leukemia virus (FELV) okanye i-feline immunodeficiency virus (FIV).
Iikati zezona ndwendwe ziphambili zeT.gondii;zizilwanyana ezanyisayo kuphela apho iToxoplasma idlula ilindle.Kwikati, uhlobo lokuzala lwe-T.gondii luhlala emathunjini kwaye i-oocysts (iifom ezinjengamaqanda ezingekavuthwa) ziphuma emzimbeni kwindle.Ii-oocysts kufuneka zibe kwindawo engqongileyo iintsuku ezi-1-5 ngaphambi kokuba zisuleleke.Iikati zidlula kuphela i-T.gondii kwilindle lazo kwiiveki ezimbalwa emva kokuba zosulelekile.Ii-oocysts ziyakwazi ukuphila iminyaka emininzi kwimo engqongileyo kwaye ziyakwazi ukumelana nezibulala-ntsholongwane ezininzi.
I-oocysts ifakwe kwimikhosi ephakathi njengeempuku kunye neentaka, okanye ezinye izilwanyana ezinjengezinja kunye nabantu, kwaye zifudukela kwimisipha kunye nengqondo.Xa ikati idla ixhoba eliphakathi elisulelekileyo (okanye inxalenye yeisilwanyana esikhulu, umzekelo, ihagu), isifunxi-gazi siphuma emathunjini ekati kwaye umjikelo wobomi unokuphindwa.
Iimpawu eziqhelekileyo zei-toxoplasmosis ibandakanya umkhuhlane, ukuphelelwa ngumdla, kunye nokutyhafa.Ezinye iimpawu zinokuthi zenzeke ngokuxhomekeke ekubeni usulelo lubukhali okanye alupheli, kwaye apho i-parasite ifumaneka khona emzimbeni.Kwimiphunga, usulelo lwe-T.gondii lunokukhokelela kwinyumoniya, eya kubangela uxinzelelo lokuphefumla olunyuka kancinci kancinci.I-Toxoplasmosis inokuchaphazela amehlo kunye nenkqubo ye-nervous central, ukuvelisa ukuvuvukala kwe-retina okanye igumbi langaphambili le-ocular, ubungakanani bomfundi obungaqhelekanga kunye nokuphendula ekukhanyeni, ukungaboni, ukulungelelaniswa, ukuvakalelwa okuphakamileyo, ukuguquka kobuntu, ukujikeleza, ukucinezela intloko, ukuxubha iindlebe. , ubunzima bokuhlafuna kunye nokuginya ukutya, ukuxhuzula, kunye nokulahlekelwa ukulawula ukuchama kunye nokukhupha.
I-Toxoplasmosis idla ngokufunyaniswa ngokusekwe kwimbali, iimpawu zokugula, kunye neziphumo zeemvavanyo zaselabhoratri ezixhasayo.Umlinganiselo we-IgG kunye ne-IgM ye-antibodies kwi-Toxoplasma gondii egazini inokunceda ukuxilonga i-toxoplasmosis.Ubukho bee-antibodies ze-IgG ezibalulekileyo kwi-T.gondii kwikati esempilweni bucebisa ukuba ikati yayikhe yasulelwa ngaphambili kwaye ngoku kusenokwenzeka ukuba ikhuselekile kwaye ayikhuphi ii-oocysts.Ubukho bee-antibodies ze-IgM ezibalulekileyo kwi-T.gondii, nangona kunjalo, zibonisa usulelo olusebenzayo lwekati.Ukungabikho kwezilwa-buhlungu ze-T.gondii zazo zombini ezi ndidi kwikati esempilweni kubonisa ukuba ikati isesichengeni sokosulelwa kwaye ngaloo ndlela ingakhupha iioocysts iveki enye ukuya kwezimbini emva kosulelo.
Akukabikho isitofu sokugonya ukuthintela usulelo lwe-T.gondii okanye i-toxoplasmosis kwiikati, abantu, okanye ezinye iintlobo.Ngoko ke, unyango ludla ngokubandakanya ikhosi yesithinteli-ntsholongwane esibizwa ngokuba yi-clindamycin.Amanye amachiza asetyenziswayo aquka i-pyrimethamine kunye ne-sulfadiazine, ezisebenza kunye ukuthintela ukuveliswa kwe-T.gondii.Unyango kufuneka luqalwe ngokukhawuleza emva kokuxilongwa kwaye luqhubeke iintsuku ezininzi emva kokuba iimpawu zilahlekile.
Usulelo olubukhali lubonakala ngokunyuka ngokukhawuleza kwe-antibody ye-IgM, elandelwa kwiiveki ezi-3-4 ngokunyuka kwe-antibody yeklasi ye-IgG.Amanqanaba e-antibody e-IgM aphezulu malunga neeveki ze-3-4 emva kokuqala kweempawu kwaye ahlala ebonakala kwiinyanga ezi-2-4.I-antibody yeklasi ye-IgG ifikelela incopho kwiiveki ezisi-7 ukuya kwezi-12, kodwa yehla kancinci kunamanqanaba e-antibody e-IgM kwaye ihlala inyukile ngaphezulu kweenyanga ezili-9-12.