Isishwankathelo | Ukufunyanwa kwe-Antibody ethile ye-AIV-H9 yentsholongwane ethile |
Umgaqo | Ikiti yovavanyo ye-H9 yoMkhuhlane weNtaka i-Elisa inokusetyenziselwa ukufumanisa i-H9 Subtype yoMkhuhlane we-Avian kwiserum yenkukhu.
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Iinjongo zokuFumana | I-antibody ye-H9 yoMkhuhlane weNtaka |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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Umkhuhlane weentaka, owaziwa ngokungekho sikweni njengomkhuhlane weentaka okanye umkhuhlane weentaka, ludidi lomkhuhlane olubangelwa ziintsholongwane eziqhelaniswe nazo.iintaka.Udidi olunomngcipheko omkhulu luphezulu
i-pathogenic avian influenza (HPAI).Umkhuhlane weentaka uyafana neumkhuhlane wehagu, umkhuhlane wenja, umkhuhlane wehashe kunye nomkhuhlane womntu njengesigulo esibangelwa ziintlobo zentsholongwane zomkhuhlane ezinazo
ilungelelaniswe kumamkeli othile.Kwiintlobo ezintathu zeentsholongwane zomkhuhlane (A,B, kwayeC), intsholongwane yomkhuhlane A yintsholongwane azoonoticusulelo ngedama lendalo phantse
ngokupheleleyo kwiintaka.Umkhuhlane weentaka, ngeenjongo ezininzi, ubhekisa kwintsholongwane yomkhuhlane A.
Oku ikhithi sebenzisa ngokungathanga ngqo ELISA indlela, ihlanjululwe I-AIV-H9HA i-antigen is iqatywe ngaphambili on i-enzyme micro kakuhle imicu. Xa uvavanya, yongeza ixutywe serum isampuli, emva incubation, if Pha is I-AIV-H9 intsholongwane ethile amajoni omzimba, it intando dibanisa nge i iqatywe ngaphambili i-antigen, lahla i engadityaniswanga i-antibody kwaye enye amacandelo nge ukuhlamba; ngoko yongeza i-enzyme dibanisa, lahla i engadityaniswanga i-enzyme dibanisa ngokuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ohlaza okwesibhakabhaka nge-Enzyme catalysis ngokuthe ngqo Umlinganiselo wesiqulatho se-antibody kwisampulu.
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |