Isishwankathelo | Ukufunyanwa kwe-Antibody ethile ye-Foot kunye ne-Mouth Disease Type I-BRU |
Umgaqo | UmGaqo woVavanyo lwe-Brucellosis(Bru) ikiti yovavanyo lwe-antibody ye-Elisa inokusetyenziselwa ukubona i-antibody ye-Brucellosis kwiserum okanye kwiplasma yeBovine neHagu. |
Iinjongo zokuFumana | I-antibody ye-BRU |
Isampulu | ISerum |
Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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I-Brucellosisiyosulela kakhului-zoonosisokubangelwa kukungena kwe-unpasteurizedubisiokanye zingaphekwanga ngokwaneleyoinyamakwizilwanyana ezosulelekileyo, okanye ukudibana ngokusondeleyo namanci azo.
Ikwabizwa ngokuba yi-undulant fever, Malta fever, kunye ne-Mediterranean fever.
Oku ikhithi sebenzisa ngokungathanga ngqo ELISA indlela, ihlanjululwe Brui-antigen is iqatywe ngaphambili on i-enzyme micro kakuhle imicu. Xa uvavanya, yongeza ixutywe serum isampuli, emva incubation, if Pha is Bru ethile amajoni omzimba, it intando dibanisa nge i iqatywe ngaphambili i-antigen, lahla i engadityaniswanga i-antibody kwaye enye amacandelo nge ukuhlamba; ngoko yongeza i-enzyme dibanisa, lahla i engadityaniswanga i-enzyme dibanisa nge
ukuhlamba. Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ohlaza okwesibhakabhaka nge-Enzyme catalysis ngokuthe ngqo Umlinganiselo wesiqulatho se-antibody kwisampulu.
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |