Isishwankathelo | Ukufunyanwa kwe-Antibody ethile ye-Foot kunye ne-Mouth Disease Type I-BRU |
Umgaqo | Ikhithi yovavanyo lwe-BRU ye-ELISA isetyenziselwa ukubona izilwa-buhlungu ze-Brucellosis kwiserum yeehagu, iinkomo, iigusha kunye neebhokhwe. |
Iinjongo zokuFumana | I-antibody ye-BRU |
Isampulu | ISerum
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Ubungakanani | Ikhithi e-1 = 192 Uvavanyo |
Uzinzo kunye noGcino | 1) Zonke ii-reagents kufuneka zigcinwe kwi-2 ~ 8℃.Musa ukuba ngumkhenkce. 2) Ubomi beShelf ziinyanga ezili-12.Sebenzisa zonke ii-reagents phambi komhla wokuphelelwa kwekhithi.
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IBrucellosisis iyosulela kakhului-zoonosisokubangelwa kukungena kwe-unpasteurizedubisiokanye zingaphekwanga ngokwaneleyoinyamakwizilwanyana ezosulelekileyo, okanye ukudibana ngokusondeleyo namanci azo.
Ikwabizwa ngokuba yi-undulant fever, Malta fever, kunye ne-Mediterranean fever.
I-BRU i-antibodyELISAikhithi yovavanyousetyenziselwa uku ukufumanisa i-Brucellosis antibodies kwi-serum yeehagu, iinkomo,sheep kunye nebhokhwe .
Sebenzisa le kit indlela ye-ELISA yokukhuphisana kwangaphambilied BRU ii-antigens kwimithombo ye-microplate.Xa uvavanya, yongeza isampuli ye-serum ehlanjululweyo kwayei-enzayim ephawulwe ngokuba yi-anti-I-BRU i-antibody ye-monoclonal, emva kokufukanyelwa, ukuba ikhona babe I-BRU i-antibody, iya kudibanisa ne-antigen eqatywe ngaphambili, i-antibody kwibhloko yesampulu indibaniselwano ye-monoclonal antibody kunye ne-antigen eqatywe ngaphambili;lahla i-enzyme edibeneyo edibeneyo kunye nokuhlamba;Yongeza i-substrate ye-TMB kumaqula amancinci, umqondiso ozuba nge-Enzyme catalysis ukwinxalenye eguqukileyo yesiqulatho se-antibody kwisampulu..
I-Reagent | Umthamo Iimvavanyo ezingama-96/192Uvavanyo | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | i-serum dilution microplate | 1ea/2ea | |
11 | Umyalelo | iipcs ezi-1 |